Analysis and Design of Digital IC's in Deep Submicron Tech. by D. Hodges, et al.,

By D. Hodges, et al.,

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39 41 44 45 45 46 49 50 52 52 55 55 58 59 62 62 64 65 66 67 69 70 70 70 71 9 40 LARS JOSEFSSON AND STEN LAGERSTEDT I. INTRODUCTION* The remarkable maturation of our understanding of the mode of action of the ribonucleases (RNases), gained from experimental work during the last decade (12,13,39,89), has made possible the identification of the characteristic steps involved in the degradation of polyribonucleotides by these enzymes. Thus a basis for an accurate methodological approach to the quantitative determination of their activities has been formed.

Introduction. . . . . . . . . , . . . . . . . . . . . . . . . . 40 . . . . . . . . . 41 11. Characteristics of Ribonuclease Activity. 111. Methods for Charact,erization... . .............. A. Preparation of Substrates. . B. Analytical Procedure of Mar th . . . . . . 2. Methods Based on the Degradation of Simple Nucleoside Derivatives . . . . . . . . . . . . . . . . . . . . . . . . . . . A. Preparation of Substrates. . B. Analytical Procedures.

B. Pardee, Biochim. el Biophys. A d a , 19,236 (1956). 73. Kurnick, N. , unpublished experiments. 74. Kurnick, N. , Cold Spring Harbor Symposia, Quant. , 12, 141 (1947). 75. Kurnick, N. , J. Gen. , 33, 243 (1950). 76. Kurnick, N. , and A. E. Mirsky, J. Gen. , 33,265 (1950). 77. Kurnick, N. , Arch. , 89, 41 (1950). 78. Kurnirk, N. , Arch. Biochem. , 43, 97 (1953). 79. Kurnirk, N. , J. Am. Chem. ,76, 417 (1954). 80. Kurnick, N. , J . Am. Chem. ,76, 4040 (1954) 81. Kurnick, N. , Henry Ford Hospital Intern.

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