By T. A. Dettlaff (auth.), T. A. Dettlaff, S. G. Vassetzky (eds.)
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Additional resources for Animal Species for Developmental Studies: Vertebrates
Thereafter the suspension of spermatozoa is replaced by filtered river or dechlorinated tap water. The layer of water in the dish should be just sufficient to cover the eggs, thus providing good conditions of gas exchange. If the eggs are incubated in the laboratory for a long time, the water should be changed not less than twice a day. In those cases where a large number of eggs is fertilized, the process is carried out as at the hatchery. The eggs are placed in a dry enamel basin and the excess of body-cavity fluid is poured off.
Dependence of the duration of one mitotic cycle during synchronous cleavage divisions ('to) on temperature in the Russian sturgeon. At l2 0 e and lower temperatures the curve calls for precision. Stage 4. First cleavage stage (Fig. 8). The first cleavage furrow divides the animal region and progresses to the darker vegetal material which is richer in yolk. Stage 5. Second cleavage stage (Fig. 8). The animal region is divided by meridional furrows into four approximately equal parts. The first cleavage furrow descends below the equator and the second cleavage furrow progresses to the darker vegetal material.
The oocytes are then washed out from the recess. 4. STRUCTURE OF MATURE GAMETES The data given below were obtained for Acipenser gUldenstiidti colchicus at the Don River and have already been published elsewhere [32, 34, 54, 57, 58]. 1. 5 mm. The color is brownish-grey. The animal region is characterized by a distinct pattern with a light polar spot in the center surrounded by a ringlike accumulation of pigment granules. There is another pigment ring at the border with the vegetal region (Fig. 7, 1 and Ian).